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KMID : 1177720140080010015
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Journal of Alternatives to Animal Experiments
2014 Volume.8 No. 1 p.15 ~ p.19
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Skin Sensitization Test of Samhwang-Sasimtang using Local Lymph Node Assay
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Kim Min-Ji
Kim Bae-Hwan
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Abstract
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The purpose of this study is to evaluate the skin sensitization ability of a natural herbal extract, Samhwang-Sasimtang, using Local Lymph Node Assay (LLNA), a alternative method to guinea pig maximization test (GPMT). A non-radioisotopic LLNA employing 5-bromo-2-deoxyuridine (BrdU) with flow cytometry (FACS) is used in this study instead of the traditional LLNA using a radioisotope, 3H-thymidine. Through the LLNA:BrdU-FCM study in Balb/c mice, Samhwang-Sasimtang extract (SSE, 0.1%, 1%, 2%) was confirmed as a non-sensitizer, because it showed lower than 3 of stimulation index (SI) which is calculated relative to the concurrent vehicle control value. 4-Phenylenediamine (PPD), a positive control, showed sensitization potential with high SIs which were greater than 3 in all tested concentrations. From this study, we also confirmed that the modified LLNA(LLNA:BrdU-FCM) in which BrdU is used in place of radioisotope incorporation is a useful alternative to animal testing for skin sensitization.
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KEYWORD
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Skin sensitization, Samhwang-Sasimtang, LLNA, BrdU, FCM
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